Hotspot

trnR-ACG-trnN-GUU

IRb · 109852-110428 · 577 bp

Back to Reaumuria

Pi 0.0355

MarkerSeek score 80.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 577
variable_sites: 20
pi: 0.0355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	708-718 bp	1.0	82.0
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	749-759 bp	1.0	81.0
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	659-669 bp	1.0	81.0
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	57.8	57.8	748-758 bp	1.0	80.2
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	663-673 bp	1.0	80.1