Hotspot

psbT-psbN

LSC · 76021-76080 · 60 bp

Back to Reaumuria

Pi 0.0667

MarkerSeek score 76.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 4
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCACCGAAAGTTC	AGACCCATTCGAAGAACACG	50.0	50.0	57.9	57.9	128 bp	1.0	80.7
psbT-psbN_p2	GAGAACCACCGAAAGTTCCA	AGACCCATTCGAAGAACACG	50.0	50.0	57.8	57.9	126 bp	1.0	79.9
psbT-psbN_p3	TCGAGAACCACCGAAAGTTC	TGTATACCGCTTTTGGGCAA	50.0	45.0	57.9	57.8	165 bp	1.0	79.2
psbT-psbN_p4	TCGAGAACCACCGAAAGTTC	TTGTATACCGCTTTTGGGCA	50.0	45.0	57.9	57.8	166 bp	1.0	79.2
psbT-psbN_p5	TCGAGAACCACCGAAAGTTC	GGTACGCTTTGTATACCGCT	50.0	50.0	57.9	57.8	174 bp	1.0	79.0