Hotspot

rpl33-rps18

LSC · 69315-69839 · 525 bp

Back to Reaumuria

Pi 0.0251

MarkerSeek score 72.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 525
variable_sites: 13
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	ATCAATTTGATCCCCCGACC	55.0	50.0	58.5	57.6	699-709 bp	1.0	75.1
rpl33-rps18_p2	CGACACAATACGCCTAGTCG	CAAACGCCTGCGAAATGATC	55.0	50.0	58.5	58.5	669-679 bp	1.0	74.4
rpl33-rps18_p3	CGACACAATACGCCTAGTCG	AAGAAAGGGTCGCTTGGATT	55.0	45.0	58.5	57.4	639-649 bp	1.0	73.5
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	AGAAAGGGTCGCTTGGATTT	55.0	45.0	58.5	57.4	638-648 bp	1.0	73.5
rpl33-rps18_p5	GACACAATACGCCTAGTCGA	ATCAATTTGATCCCCCGACC	50.0	50.0	57.2	57.6	698-708 bp	1.0	72.6