Hotspot

petL-petG

LSC · 67410-67600 · 191 bp

Back to Reaumuria

Pi 0.0628

MarkerSeek score 79.70

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 12
pi: 0.0628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	GGTTTCCTACTAGCGGCTTT	AGGTCCAATTGATCACCACG	50.0	50.0	57.6	57.9	373-376 bp	1.0	78.5
petL-petG_p2	GGTTTCCTACTAGCGGCTTT	TATGCGGTCACGAATAAGCC	50.0	50.0	57.6	58.1	340-343 bp	1.0	78.5
petL-petG_p3	GGTTTCCTACTAGCGGCTTT	TCACGAATAAGCCAGCCAAA	50.0	45.0	57.6	57.8	333-336 bp	1.0	78.1
petL-petG_p4	GGTTTCCTACTAGCGGCTTT	ACGAATAAGCCAGCCAAAGT	50.0	45.0	57.6	57.8	331-334 bp	1.0	78.1
petL-petG_p5	GGTTTCCTACTAGCGGCTTT	GGTCCAATTGATCACCACGT	50.0	50.0	57.6	58.2	372-375 bp	1.0	78.0