Hotspot

psbE-petL

LSC · 66091-67313 · 1223 bp

Back to Reaumuria

Pi 0.0275

MarkerSeek score 62.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 26
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAAGCCGCTAGTAGGAAACC	50.0	50.0	57.8	57.6	1025-1294 bp	1.0	81.4
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AAAGCCGCTAGTAGGAAACC	55.0	50.0	58.2	57.6	1020-1289 bp	1.0	81.0
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AAAGCCGCTAGTAGGAAACC	50.0	50.0	58.4	57.6	1018-1287 bp	1.0	79.8
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	CCGCTAGTAGGAAACCGAAA	50.0	50.0	57.8	57.3	1021-1290 bp	1.0	79.3
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	CCGCTAGTAGGAAACCGAAA	55.0	50.0	58.2	57.3	1016-1285 bp	1.0	78.9