Hotspot

trnG-GCC-trnfM-CAU

LSC · 37579-37752 · 174 bp

Back to Reaumuria

Pi 0.0575

MarkerSeek score 74.10

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 10
pi: 0.0575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	251-260 bp	1.0	81.5
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	245-254 bp	1.0	81.0
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	244-253 bp	1.0	80.4
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	249-258 bp	1.0	80.4
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	CACGGGTTCAAATCCTGTCT	50.0	50.0	57.8	57.8	243-252 bp	1.0	80.4