Hotspot

trnT-GGU-psbD

LSC · 32576-33879 · 1304 bp

Back to Reaumuria

Pi 0.0261
MarkerSeek score 68.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1304
variable_sites
29
pi
0.0261
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1284-1468 bp 1.0 83.1
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1287-1471 bp 1.0 82.9
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1286-1470 bp 1.0 82.7
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TAGACCGGACCAACCTACAA 50.0 50.0 58.1 57.7 1289-1473 bp 1.0 82.5
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG GAGCAATAGACCGGACCAAC 55.0 55.0 58.1 58.3 1292-1476 bp 1.0 82.4