Hotspot

trnC-GCA-petN

LSC · 28183-29060 · 878 bp

Back to Reaumuria

Pi 0.0182

MarkerSeek score 69.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 15
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	882-934 bp	1.0	83.0
trnC-GCA-petN_p2	TTTATCGTTTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.7	58.2	936-988 bp	1.0	81.9
trnC-GCA-petN_p3	ATTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	943-995 bp	1.0	81.3
trnC-GCA-petN_p4	TAAGGCGGAGGATTGCAAAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.6	58.2	907-959 bp	1.0	80.6
trnC-GCA-petN_p5	TTTATCGTTTTGGCGGCATG	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.7	57.6	997-1049 bp	1.0	80.2