Hotspot

atpH-atpI

LSC · 14029-14658 · 630 bp

Back to Reaumuria

Pi 0.0181
MarkerSeek score 69.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
630
variable_sites
11
pi
0.0181
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GCCAACTCCAGGTCCAATAG TTTGCAACTTTAGCTGCAGC 55.0 45.0 58.0 57.9 743-765 bp 1.0 82.0
atpH-atpI_p2 GCCAACTCCAGGTCCAATAG CCCGTCATGTTCCTTGGATT 55.0 50.0 58.0 57.9 791-813 bp 1.0 81.9
atpH-atpI_p3 GCCAACTCCAGGTCCAATAG CGAATCCATGGAGGGTCATC 55.0 55.0 58.0 57.8 714-736 bp 1.0 81.9
atpH-atpI_p4 CAGCAGCAATAACAGAAGCG TTTGCAACTTTAGCTGCAGC 50.0 45.0 57.8 57.9 702-724 bp 1.0 81.2
atpH-atpI_p5 CAGCAGCAATAACAGAAGCG CCCGTCATGTTCCTTGGATT 50.0 50.0 57.8 57.9 750-772 bp 1.0 81.1