Hotspot

rps16-trnQ-UUG

LSC · 5984-7009 · 1026 bp

Back to Reaumuria

Pi 0.0541

MarkerSeek score 77.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1026
variable_sites: 54
pi: 0.0541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCCGCACGATAGACTTTTGA	GAACAGCGGTATAACTGGCA	50.0	50.0	58.0	58.0	1967-1990 bp	1.0	84.4
rps16-trnQ-UUG_p2	TCCCGCACGATAGACTTTTG	GAACAGCGGTATAACTGGCA	50.0	50.0	58.0	58.0	1968-1991 bp	1.0	84.4
rps16-trnQ-UUG_p3	CCCGCACGATAGACTTTTGA	CGAAAACTTACCGCAGCTTG	50.0	50.0	58.0	58.0	2011-2034 bp	1.0	84.0
rps16-trnQ-UUG_p4	TGGCAGCAACATACCCTTTT	GAACAGCGGTATAACTGGCA	45.0	50.0	58.0	58.0	2026 bp	0.5	59.8
rps16-trnQ-UUG_p5	AACCCTAGATTCTTGCCCCT	GAACAGCGGTATAACTGGCA	50.0	50.0	58.0	58.0	1811 bp	0.5	59.7