Hotspot

trnK-UUU-rps16

LSC · 4151-4854 · 704 bp

Back to Reaumuria

Pi 0.0468

MarkerSeek score 77.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 31
pi: 0.0468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCTACCGATGATGTGGACGA	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.0	58.0	868-912 bp	1.0	86.6
trnK-UUU-rps16_p2	TTCAGGATCAGTCGTGGTCT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.1	58.0	895-939 bp	1.0	86.3
trnK-UUU-rps16_p3	TCTACCGATGATGTGGACGA	GCCGTACGAGGAGAAAACTT	50.0	50.0	58.0	57.9	1058-1102 bp	1.0	85.2
trnK-UUU-rps16_p4	TCTACCGATGATGTGGACGA	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	1464-1500 bp	1.0	85.1
trnK-UUU-rps16_p5	TTCAGGATCAGTCGTGGTCT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1491-1527 bp	1.0	84.7