Hotspot

trnH-GUG-psbA

LSC · 68-294 · 227 bp

Back to Reaumuria

Pi 0.0284

MarkerSeek score 70.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 6
pi: 0.0284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TGGGCTGACATCATTAACCG	55.0	50.0	58.1	58.0	347-362 bp	1.0	83.6
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GGGCTGACATCATTAACCGT	55.0	50.0	58.1	58.0	346-361 bp	1.0	83.6
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TGGGCTGACATCATTAACCG	50.0	50.0	57.9	58.0	361-376 bp	1.0	83.4
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	GGGCTGACATCATTAACCGT	50.0	50.0	57.9	58.0	360-375 bp	1.0	83.4
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	TGGGCTGACATCATTAACCG	50.0	50.0	57.9	58.0	360-375 bp	1.0	83.4