Hotspot

ycf1

SSC · 124868-130435 · 5568 bp

Back to Rauvolfia

Pi 0.0128

MarkerSeek score 53.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 101
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAAAGGGGAGAATGCACGT	ATCCGCCCAGTTTGTCAATT	45.0	45.0	58.0	58.1	848-983 bp	1.0	85.9
ycf1_p2	AAAAGGGGAGAATGCACGTT	ATCCGCCCAGTTTGTCAATT	45.0	45.0	58.0	58.1	847-982 bp	1.0	85.9
ycf1_p3	AAAGGGGAGAATGCACGTTT	ATCCGCCCAGTTTGTCAATT	45.0	45.0	58.0	58.1	846-981 bp	1.0	85.9
ycf1_p4	ATTTGTATGACCATCGGGGC	ATCCGCCCAGTTTGTCAATT	50.0	45.0	58.0	58.1	687 bp	0.666667	69.5
ycf1_p5	AGCGCGCATAGAACCTTTAA	ATCCGCCCAGTTTGTCAATT	45.0	45.0	58.0	58.1	923 bp	0.666667	68.6