Hotspot

atpB-rbcL

LSC · 55727-56498 · 772 bp

Back to Rauvolfia

Pi 0.00864

MarkerSeek score 63.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 10
pi: 0.00864
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGGTTGTTTTT	CACCAGCTTTGAATCCAACAC	45.0	47.6	58.0	58.0	881-889 bp	1.0	74.1
atpB-rbcL_p2	ATACGCCCCAGGTTGTTTTT	ACCAGCTTTGAATCCAACACT	45.0	42.9	58.0	58.1	880-888 bp	1.0	73.8
atpB-rbcL_p3	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	852-860 bp	1.0	73.5
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	851-859 bp	1.0	73.2
atpB-rbcL_p5	ATGATTTGGACGATACGCCC	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	893-901 bp	1.0	73.1