Hotspot

ndhC-trnV-UAC

LSC · 51383-52685 · 1303 bp

Back to Rauvolfia

Pi 0.0253
MarkerSeek score 75.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1303
variable_sites
49
pi
0.0253
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1434-1485 bp 1.0 84.0
ndhC-trnV-UAC_p2 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 1451-1502 bp 1.0 82.4
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1446-1497 bp 1.0 82.4
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1433-1484 bp 1.0 81.3
ndhC-trnV-UAC_p5 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 1447-1498 bp 1.0 81.1