Hotspot

rps4-trnT-UGU

LSC · 46696-47056 · 361 bp

Back to Rauvolfia

Pi 0.0591

MarkerSeek score 65.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 361
variable_sites: 32
pi: 0.0591
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCAGCCGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	55.6	55.0	58.4	57.9	471-489 bp	1.0	65.8
rps4-trnT-UGU_p2	CCCAGCCGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	55.6	50.0	58.4	57.8	439-457 bp	1.0	65.3
rps4-trnT-UGU_p3	CCCAGCCGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	55.6	55.0	58.4	57.5	479-497 bp	1.0	62.7
rps4-trnT-UGU_p4	CCCAGCCGGCGTATTTTT	CAGAGGTTAGAGCATCGCAT	55.6	50.0	58.4	57.5	469-487 bp	1.0	62.7
rps4-trnT-UGU_p5	CCCAGCCGGCGTATTTTT	TCAGAGGTTAGAGCATCGCA	55.6	50.0	58.4	58.5	470-488 bp	1.0	62.6