Hotspot

trnE-UUC-trnT-GGU

LSC · 31331-32237 · 907 bp

Back to Rauvolfia

Pi 0.0339

MarkerSeek score 72.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 46
pi: 0.0339
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGAGATGTCCTGGACCAC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	996-1023 bp	1.0	83.5
trnE-UUC-trnT-GGU_p2	GAGAGATGTCCTGGACCACT	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	995-1022 bp	1.0	83.5
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	1011-1038 bp	1.0	83.2
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1048-1075 bp	1.0	83.1
trnE-UUC-trnT-GGU_p5	GTCCTGGACCACTAGACGAT	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	988-1015 bp	1.0	82.8