Hotspot

rpoB-trnC-GCA

LSC · 26464-27704 · 1241 bp

Back to Rauvolfia

Pi 0.0118

MarkerSeek score 66.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 22
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAGTTCTTCCGTCAAACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1408-1422 bp	1.0	80.9
rpoB-trnC-GCA_p2	CGTCAAACCCCGATCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.3	58.1	1399-1413 bp	1.0	79.0
rpoB-trnC-GCA_p3	AAGTTCTTCCGTCAAACCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1390-1404 bp	1.0	78.8
rpoB-trnC-GCA_p4	CGTCAAACCCCGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.3	58.4	1381-1395 bp	1.0	76.8
rpoB-trnC-GCA_p5	AAGTTCTTCCGTCAAACCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	58.0	58.8	1427-1441 bp	1.0	75.4