Hotspot

trnR-UCU-atpA

LSC · 9523-9653 · 131 bp

Back to Rauvolfia

Pi 0.0178

MarkerSeek score 67.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 3
pi: 0.0178
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATCCGAATGAAAAGCGTCCA	AAGACATTCACCGAGGAAGC	45.0	50.0	57.9	57.9	287-297 bp	1.0	81.9
trnR-UCU-atpA_p2	TCCGAATGAAAAGCGTCCAT	AAGACATTCACCGAGGAAGC	45.0	50.0	57.9	57.9	286-296 bp	1.0	81.9
trnR-UCU-atpA_p3	ATCCGAATGAAAAGCGTCCA	CCAAGACATTCACCGAGGAA	45.0	50.0	57.9	57.5	289-299 bp	1.0	78.9
trnR-UCU-atpA_p4	TCCGAATGAAAAGCGTCCAT	CCAAGACATTCACCGAGGAA	45.0	50.0	57.9	57.5	288-298 bp	1.0	78.9
trnR-UCU-atpA_p5	TCCGAATGAAAAGCGTCCAT	TCAGGAACAAATGGACCGTT	45.0	45.0	57.9	57.4	242-252 bp	1.0	78.1