Hotspot

rps16-trnQ-UUG

LSC · 5735-6977 · 1243 bp

Back to Rauvolfia

Pi 0.0215

MarkerSeek score 63.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1243
variable_sites: 40
pi: 0.0215
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TTCTACTCGAGCTCCATCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1441-1493 bp	1.0	83.4
rps16-trnQ-UUG_p2	TTCTACTCGAGCTCCATCGT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	2002-2066 bp	1.0	82.6
rps16-trnQ-UUG_p3	AACTTGCTCGAATGGGATCC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	2118-2182 bp	1.0	82.6
rps16-trnQ-UUG_p4	AACTTGCTCGAATGGGATCC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2123-2187 bp	1.0	82.6
rps16-trnQ-UUG_p5	TTCTACTCGAGCTCCATCGT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2007-2071 bp	1.0	82.5