Hotspot

ycf1

SSC · 126566-132199 · 5634 bp

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Pi 0.0127

MarkerSeek score 38.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5634
variable_sites: 142
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGACAGACGAATGGGTTC	AAACCAAGGAAATCGCGGAT	50.0	45.0	57.5	58.2	378 bp	1.0	80.3
ycf1_p2	ACAGACAGACGAATGGGTTC	GGCCGAAGAGATCTTGGATC	50.0	55.0	57.5	57.8	407 bp	1.0	80.2
ycf1_p3	ACAGACAGACGAATGGGTTC	AGGCCGAAGAGATCTTGGAT	50.0	50.0	57.5	58.2	408 bp	1.0	79.8
ycf1_p4	ACAGACAGACGAATGGGTTC	CAAAACCAAGGAAATCGCGG	50.0	50.0	57.5	58.3	380 bp	1.0	79.2
ycf1_p5	ACAGACAGACGAATGGGTTC	CCTGGACCAAAATGGATCCC	50.0	55.0	57.5	58.2	835 bp	1.0	77.9