Hotspot

rps15-ycf1

SSC · 126165-126565 · 401 bp

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Pi 0.0129
MarkerSeek score 55.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
401
variable_sites
10
pi
0.0129
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ATTCAGCAGACCCCTTGTTG TGGAAGCAATTTCAGTATGTTAAGG 50.0 36.0 58.1 57.8 518-523 bp 1.0 42.3
rps15-ycf1_p2 ATTCAGCAGACCCCTTGTTG TGGAAGCAATTTCAGTATGT 50.0 35.0 58.1 52.8 518-523 bp 1.0 42.3
rps15-ycf1_p3 ATTCAGCAGACCCCTTGTTG TGGAAGCAATTTCAGTATGTT 50.0 33.3 58.1 53.7 518-523 bp 1.0 42.3
rps15-ycf1_p4 GCAGACCCCTTGTTGTCTTT TGGAAGCAATTTCAGTATGTTAAGG 50.0 36.0 58.3 57.8 513-518 bp 1.0 42.3
rps15-ycf1_p5 ATTCAGCAGACCCCTTGTTG GGAAGCAATTTCAGTATGTTAAGGA 50.0 36.0 58.1 57.6 517-522 bp 1.0 42.3