Hotspot

ndhF-rpl32

SSC · 114786-115970 · 1185 bp

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Pi 0.012

MarkerSeek score 65.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1185
variable_sites: 28
pi: 0.012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1370-1386 bp	1.0	80.1
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1368-1384 bp	1.0	80.1
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1369-1385 bp	1.0	80.1
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1381-1397 bp	1.0	79.0
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	GACAACGCTTTTAAGGCTGC	45.0	50.0	58.0	58.3	1379-1395 bp	1.0	78.9