Hotspot

rpl16-rps3

LSC · 84028-85174 · 1147 bp

Back to Raukaua

Pi 0.0125

MarkerSeek score 60.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 26
pi: 0.0125
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	TGGATATTTATAGACGGGGAAGA	50.0	39.1	58.4	56.1	1222-1241 bp	1.0	43.3
rpl16-rps3_p2	AGGTTCAAGTGCCTGAAGAG	TGGATATTTATAGACGGGGAAGA	50.0	39.1	57.4	56.1	1224-1243 bp	1.0	43.3
rpl16-rps3_p3	GTTCAAGTGCCTGAAGAGCA	TGTTCCTATACAGTTCGAACT	50.0	38.1	58.4	54.1	1270-1289 bp	1.0	43.2
rpl16-rps3_p4	AGGTTCAAGTGCCTGAAGAG	TGTTCCTATACAGTTCGAACT	50.0	38.1	57.4	54.1	1272-1291 bp	1.0	43.2
rpl16-rps3_p5	GTTCAAGTGCCTGAAGAGCA	TGGAGTATTAGGCATCAAAATTTGG	50.0	36.0	58.4	57.9	1244-1263 bp	1.0	43.2