Hotspot

rps11-rpl36

LSC · 81880-81980 · 101 bp

Back to Raukaua

Pi 0.0264

MarkerSeek score 59.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 101
variable_sites: 5
pi: 0.0264
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CCTACGTGAACCACTTCTCG	GACTAATTCGTAGGCGGGGA	55.0	55.0	58.0	59.0	208-217 bp	1.0	74.0
rps11-rpl36_p2	CTACGTGAACCACTTCTCGG	GACTAATTCGTAGGCGGGGA	55.0	55.0	58.0	59.0	207-216 bp	1.0	74.0
rps11-rpl36_p3	GAACCAATACGCCCATTCCT	GACTAATTCGTAGGCGGGGA	50.0	55.0	57.9	59.0	225-234 bp	1.0	73.6
rps11-rpl36_p4	AATACGCCCATTCCTACGTG	GACTAATTCGTAGGCGGGGA	50.0	55.0	57.8	59.0	220-229 bp	1.0	72.2
rps11-rpl36_p5	CAATACGCCCATTCCTACGT	GACTAATTCGTAGGCGGGGA	50.0	55.0	57.8	59.0	221-230 bp	1.0	72.2