Hotspot

psaJ-rpl33

LSC · 69581-70026 · 446 bp

Back to Raukaua

Pi 0.00262

MarkerSeek score 56.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 2
pi: 0.00262
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	CACCGTTTCGAACACAACTG	45.0	50.0	58.0	57.9	516-548 bp	1.0	83.4
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	CACCGTTTCGAACACAACTG	45.0	50.0	58.1	57.9	507-539 bp	1.0	83.0
psaJ-rpl33_p3	TTCCCAGATGCGTTGACATT	CACCGTTTCGAACACAACTG	45.0	50.0	57.8	57.9	514-546 bp	1.0	82.0
psaJ-rpl33_p4	TTTCCCAGATGCGTTGACAT	CACCGTTTCGAACACAACTG	45.0	50.0	57.8	57.9	515-547 bp	1.0	82.0
psaJ-rpl33_p5	TTTTCCCAGATGCGTTGACA	ACACCGTTTCGAACACAACT	45.0	45.0	58.0	58.3	517-549 bp	1.0	81.9