Hotspot

trnS-GCT-trnR-TCT

LSC · 9418-11085 · 1668 bp

Back to Raukaua

Pi 0.00722

MarkerSeek score 62.10

Primer available yes

Label trnS-GCT-trnR-TCT

Feature Metadata

label_name: trnS-GCT-trnR-TCT
feature_type: igs
region: LSC
length_bp: 1668
variable_sites: 24
pi: 0.00722
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnR-TCT_p1	ATTAGCAATCCGCCGCTTTA	TCTTTCAAAAGGGCTTCCGA	45.0	45.0	58.0	57.4	1828-1843 bp	1.0	76.5
trnS-GCT-trnR-TCT_p2	ACGGAAAGAGAGGGATTCGA	TCTTTCAAAAGGGCTTCCGA	50.0	45.0	58.2	57.4	1877-1892 bp	1.0	75.5
trnS-GCT-trnR-TCT_p3	GCTTTAGTCCACTCAGCCAT	TCTTTCAAAAGGGCTTCCGA	50.0	45.0	57.6	57.4	1814-1829 bp	1.0	73.5
trnS-GCT-trnR-TCT_p4	ATTAGCAATCCGCCGCTTTA	TCTTTCAAAAGGGCTTCCGAA	45.0	42.9	58.0	58.1	1828-1843 bp	1.0	73.2
trnS-GCT-trnR-TCT_p5	AGTCCACTCAGCCATCTCTC	TCTTTCAAAAGGGCTTCCGA	55.0	45.0	58.5	57.4	1809-1824 bp	1.0	72.7