Hotspot

rps16-trnQ-TTG

LSC · 6318-8052 · 1735 bp

Back to Raukaua

Pi 0.00965
MarkerSeek score 58.80
Primer available yes
Label rps16-trnQ-TTG

Feature Metadata

label_name
rps16-trnQ-TTG
feature_type
igs
region
LSC
length_bp
1735
variable_sites
33
pi
0.00965
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-TTG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1804-1825 bp 1.0 80.3
rps16-trnQ-TTG_p2 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1819-1840 bp 1.0 78.4
rps16-trnQ-TTG_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1818-1839 bp 1.0 78.4
rps16-trnQ-TTG_p4 CCAAGAGCACCTTCATTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1866-1896 bp 1.0 77.3
rps16-trnQ-TTG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1808-1829 bp 1.0 75.6