primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	trnH-GTG-psbA	1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	76	484	1066-1102	1.000000	82.9
trnH-GTG-psbA_p2	trnH-GTG-psbA	2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	76	484	1072-1108	1.000000	82.9
trnH-GTG-psbA_p3	trnH-GTG-psbA	3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	76	484	1067-1103	1.000000	82.9
trnH-GTG-psbA_p4	trnH-GTG-psbA	4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	76	484	1068-1104	1.000000	82.9
trnH-GTG-psbA_p5	trnH-GTG-psbA	5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	76	484	1080-1116	1.000000	82.8
rps16-trnQ-TTG_p1	rps16-trnQ-TTG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6318	8052	1804-1825	1.000000	80.3
rps16-trnQ-TTG_p2	rps16-trnQ-TTG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6318	8052	1819-1840	1.000000	78.4
rps16-trnQ-TTG_p3	rps16-trnQ-TTG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6318	8052	1818-1839	1.000000	78.4
rps16-trnQ-TTG_p4	rps16-trnQ-TTG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6318	8052	1866-1896	1.000000	77.3
rps16-trnQ-TTG_p5	rps16-trnQ-TTG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6318	8052	1808-1829	1.000000	75.6
trnS-GCT-trnR-TCT_p1	trnS-GCT-trnR-TCT	1	ATTAGCAATCCGCCGCTTTA	TCTTTCAAAAGGGCTTCCGA	20	20	45.0	45.0	58.0	57.4	0.0	2.8	0.687738	9418	11085	1828-1843	1.000000	76.5
trnS-GCT-trnR-TCT_p2	trnS-GCT-trnR-TCT	2	ACGGAAAGAGAGGGATTCGA	TCTTTCAAAAGGGCTTCCGA	20	20	50.0	45.0	58.2	57.4	0.0	2.8	0.800880	9418	11085	1877-1892	1.000000	75.5
trnS-GCT-trnR-TCT_p3	trnS-GCT-trnR-TCT	3	GCTTTAGTCCACTCAGCCAT	TCTTTCAAAAGGGCTTCCGA	20	20	50.0	45.0	57.6	57.4	0.9	2.8	1.059480	9418	11085	1814-1829	1.000000	73.5
trnS-GCT-trnR-TCT_p4	trnS-GCT-trnR-TCT	4	ATTAGCAATCCGCCGCTTTA	TCTTTCAAAAGGGCTTCCGAA	20	21	45.0	42.9	58.0	58.1	0.0	2.8	1.099368	9418	11085	1828-1843	1.000000	73.2
trnS-GCT-trnR-TCT_p5	trnS-GCT-trnR-TCT	5	AGTCCACTCAGCCATCTCTC	TCTTTCAAAAGGGCTTCCGA	20	20	55.0	45.0	58.5	57.4	0.0	2.8	1.165493	9418	11085	1809-1824	1.000000	72.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	20	20	50.0	55.0	57.8	57.9	0.0	2.2	0.240678	28142	29446	1319-1351	1.000000	80.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256813	28142	29446	1359-1391	1.000000	80.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATCTCGGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.8	58.4	0.0	14.6	0.530176	28142	29446	1341-1373	1.000000	78.0
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCCCTCATTTCCATCTCGGA	CCTTACCACTCGGCCATATC	20	20	50.0	55.0	57.8	57.5	0.0	2.2	0.632051	28142	29446	1320-1352	1.000000	77.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCCCTCATTTCCATCTCGGA	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	57.8	58.8	0.0	0.0	0.939864	28142	29446	1378-1410	1.000000	74.7
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGATATGGCCGAGTGGTAAG	TTAAAGCAGCCCAAGCAAGA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.072037	29518	30207	791-807	1.000000	81.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	29518	30207	751-767	1.000000	81.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	CGATATGGCCGAGTGGTAAG	CAGCCCAAGCAAGACTTACT	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.257120	29518	30207	785-801	1.000000	79.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	29518	30207	745-761	1.000000	79.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	29518	30207	769-785	1.000000	78.8
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.051764	67130	68299	1306-1315	1.000000	80.8
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.057305	67130	68299	1308-1317	1.000000	80.8
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	67130	68299	1229-1238	1.000000	80.0
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	67130	68299	1231-1240	1.000000	80.0
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	67130	68299	1315-1324	1.000000	79.8
psaJ-rpl33_p1	psaJ-rpl33	1	TTTTCCCAGATGCGTTGACA	CACCGTTTCGAACACAACTG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.111267	69581	70026	516-548	1.000000	83.4
psaJ-rpl33_p2	psaJ-rpl33	2	ATGCGTTGACATTCCCCTTT	CACCGTTTCGAACACAACTG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.162176	69581	70026	507-539	1.000000	83.0
psaJ-rpl33_p3	psaJ-rpl33	3	TTCCCAGATGCGTTGACATT	CACCGTTTCGAACACAACTG	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.284359	69581	70026	514-546	1.000000	82.0
psaJ-rpl33_p4	psaJ-rpl33	4	TTTCCCAGATGCGTTGACAT	CACCGTTTCGAACACAACTG	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.284359	69581	70026	515-547	1.000000	82.0
psaJ-rpl33_p5	psaJ-rpl33	5	TTTTCCCAGATGCGTTGACA	ACACCGTTTCGAACACAACT	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.296451	69581	70026	517-549	1.000000	81.9
rps18-rpl20_p1	rps18-rpl20	1	GAACTGCTGGTCTTAGAGCC	GAAAGAATCCACCGGAATAA	20	20	55.0	40.0	58.0	53.0	9.0	9.5	5.046806	70700	70963	321-322	1.000000	41.0
rps18-rpl20_p2	rps18-rpl20	2	TTGAAAGAACCGAGTCGACC	GAAAGAATCCACCGGAATAA	20	20	50.0	40.0	57.9	53.0	15.6	9.5	5.174149	70700	70963	345-346	1.000000	40.9
rps18-rpl20_p3	rps18-rpl20	3	GAACTGCTGGTCTTAGAGCC	GCTTCTGAATCGTAAAATACTTGC	20	24	55.0	37.5	58.0	57.0	9.0	0.0	5.034821	70700	70963	414-415	1.000000	40.8
rps18-rpl20_p4	rps18-rpl20	4	GAACTGCTGGTCTTAGAGCC	TCTGAATCGTAAAATACTTGCACA	20	24	55.0	33.3	58.0	57.0	9.0	0.0	5.053172	70700	70963	411-412	1.000000	40.8
rps18-rpl20_p5	rps18-rpl20	5	TTGAAAGAACCGAGTCGACC	GCTTCTGAATCGTAAAATACTTGC	20	24	50.0	37.5	57.9	57.0	15.6	0.0	5.162165	70700	70963	438-439	1.000000	40.7
rps11-rpl36_p1	rps11-rpl36	1	CCTACGTGAACCACTTCTCG	GACTAATTCGTAGGCGGGGA	20	20	55.0	55.0	58.0	59.0	0.0	0.0	0.986319	81880	81980	208-217	1.000000	74.0
rps11-rpl36_p2	rps11-rpl36	2	CTACGTGAACCACTTCTCGG	GACTAATTCGTAGGCGGGGA	20	20	55.0	55.0	58.0	59.0	0.0	0.0	0.986319	81880	81980	207-216	1.000000	74.0
rps11-rpl36_p3	rps11-rpl36	3	GAACCAATACGCCCATTCCT	GACTAATTCGTAGGCGGGGA	20	20	50.0	55.0	57.9	59.0	0.0	0.0	1.017643	81880	81980	225-234	1.000000	73.6
rps11-rpl36_p4	rps11-rpl36	4	AATACGCCCATTCCTACGTG	GACTAATTCGTAGGCGGGGA	20	20	50.0	55.0	57.8	59.0	0.0	0.0	1.199161	81880	81980	220-229	1.000000	72.2
rps11-rpl36_p5	rps11-rpl36	5	CAATACGCCCATTCCTACGT	GACTAATTCGTAGGCGGGGA	20	20	50.0	55.0	57.8	59.0	0.0	0.0	1.199161	81880	81980	221-230	1.000000	72.2
rpl16-rps3_p1	rpl16-rps3	1	GTTCAAGTGCCTGAAGAGCA	TGGATATTTATAGACGGGGAAGA	20	23	50.0	39.1	58.4	56.1	0.0	0.0	5.347191	84028	85174	1222-1241	1.000000	43.3
rpl16-rps3_p2	rpl16-rps3	2	AGGTTCAAGTGCCTGAAGAG	TGGATATTTATAGACGGGGAAGA	20	23	50.0	39.1	57.4	56.1	0.0	0.0	5.502853	84028	85174	1224-1243	1.000000	43.3
rpl16-rps3_p3	rpl16-rps3	3	GTTCAAGTGCCTGAAGAGCA	TGTTCCTATACAGTTCGAACT	20	21	50.0	38.1	58.4	54.1	0.0	24.9	5.346970	84028	85174	1270-1289	1.000000	43.2
rpl16-rps3_p4	rpl16-rps3	4	AGGTTCAAGTGCCTGAAGAG	TGTTCCTATACAGTTCGAACT	20	21	50.0	38.1	57.4	54.1	0.0	24.9	5.502631	84028	85174	1272-1291	1.000000	43.2
rpl16-rps3_p5	rpl16-rps3	5	GTTCAAGTGCCTGAAGAGCA	TGGAGTATTAGGCATCAAAATTTGG	20	25	50.0	36.0	58.4	57.9	0.0	5.8	5.513505	84028	85174	1244-1263	1.000000	43.2
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	20	20	50.0	50.0	58.0	58.2	17.5	16.6	0.199227	114786	115970	1370-1386	1.000000	80.1
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	17.5	16.6	0.210516	114786	115970	1368-1384	1.000000	80.1
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	17.5	16.6	0.210516	114786	115970	1369-1385	1.000000	80.1
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	20	20	50.0	50.0	58.0	58.3	17.5	0.0	0.345950	114786	115970	1381-1397	1.000000	79.0
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	GACAACGCTTTTAAGGCTGC	20	20	45.0	50.0	58.0	58.3	17.5	0.0	0.357239	114786	115970	1379-1395	1.000000	78.9
rpl32-trnL-TAG_p1	rpl32-trnL-TAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	116136	116959	335-951	1.000000	82.3
rpl32-trnL-TAG_p2	rpl32-trnL-TAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	116136	116959	334-952	1.000000	82.3
rpl32-trnL-TAG_p3	rpl32-trnL-TAG	3	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	116136	116959	334-961	1.000000	81.4
rpl32-trnL-TAG_p4	rpl32-trnL-TAG	4	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.321366	116136	116959	324-940	1.000000	81.2
rpl32-trnL-TAG_p5	rpl32-trnL-TAG	5	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.322736	116136	116959	323-941	1.000000	81.2
rps15-ycf1_p1	rps15-ycf1	1	ATTCAGCAGACCCCTTGTTG	TGGAAGCAATTTCAGTATGTTAAGG	20	25	50.0	36.0	58.1	57.8	0.0	0.0	5.241735	126165	126565	518-523	1.000000	42.3
rps15-ycf1_p2	rps15-ycf1	2	ATTCAGCAGACCCCTTGTTG	TGGAAGCAATTTCAGTATGT	20	20	50.0	35.0	58.1	52.8	0.0	0.0	5.332625	126165	126565	518-523	1.000000	42.3
rps15-ycf1_p3	rps15-ycf1	3	ATTCAGCAGACCCCTTGTTG	TGGAAGCAATTTCAGTATGTT	20	21	50.0	33.3	58.1	53.7	0.0	0.0	5.400531	126165	126565	518-523	1.000000	42.3
rps15-ycf1_p4	rps15-ycf1	4	GCAGACCCCTTGTTGTCTTT	TGGAAGCAATTTCAGTATGTTAAGG	20	25	50.0	36.0	58.3	57.8	0.0	0.0	5.464466	126165	126565	513-518	1.000000	42.3
rps15-ycf1_p5	rps15-ycf1	5	ATTCAGCAGACCCCTTGTTG	GGAAGCAATTTCAGTATGTTAAGGA	20	25	50.0	36.0	58.1	57.6	0.0	0.0	5.476007	126165	126565	517-522	1.000000	42.3
ycf1_p1	ycf1	1	ACAGACAGACGAATGGGTTC	AAACCAAGGAAATCGCGGAT	20	20	50.0	45.0	57.5	58.2	0.0	6.1	0.623178	127174	127323	378-378	1.000000	80.3
ycf1_p2	ycf1	2	ACAGACAGACGAATGGGTTC	GGCCGAAGAGATCTTGGATC	20	20	50.0	55.0	57.5	57.8	0.0	6.2	0.619685	127174	127323	407-407	1.000000	80.2
ycf1_p3	ycf1	3	ACAGACAGACGAATGGGTTC	AGGCCGAAGAGATCTTGGAT	20	20	50.0	50.0	57.5	58.2	0.0	6.2	0.657432	127174	127323	408-408	1.000000	79.8
ycf1_p4	ycf1	4	ACAGACAGACGAATGGGTTC	CAAAACCAAGGAAATCGCGG	20	20	50.0	50.0	57.5	58.3	0.0	6.1	0.760378	127174	127323	380-380	1.000000	79.2
ycf1_p5	ycf1	5	ACAGACAGACGAATGGGTTC	CCTGGACCAAAATGGATCCC	20	20	50.0	55.0	57.5	58.2	0.0	9.9	0.676863	127174	127323	835-835	1.000000	77.9