Hotspot

ycf1

SSC · 122878-128181 · 5304 bp

Back to Raphanus

Pi 0.00151

MarkerSeek score 30.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5304
variable_sites: 12
pi: 0.00151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCGTTATGCGGTTCTTTT	TCAGAACCCCAAGAAGAAAA	40.0	40.0	55.4	54.4	286 bp	1.0	41.1
ycf1_p2	TCTCGTTATGCGGTTCTTTT	TCAGAACCCCAAGAAGAAAAA	40.0	38.1	55.4	55.2	286 bp	1.0	41.1
ycf1_p3	AAATCTCGTTATGCGGTTCT	TCAGAACCCCAAGAAGAAAA	40.0	40.0	55.2	54.4	289 bp	1.0	41.1
ycf1_p4	AATCTCGTTATGCGGTTCTT	TCAGAACCCCAAGAAGAAAA	40.0	40.0	55.2	54.4	288 bp	1.0	41.1
ycf1_p5	ATCTCGTTATGCGGTTCTTT	TCAGAACCCCAAGAAGAAAA	40.0	40.0	55.2	54.4	287 bp	1.0	41.1