Hotspot

psbH

LSC · 73506-73727 · 222 bp

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Pi 0
MarkerSeek score 21.70
Primer available yes
Label psbH

Feature Metadata

label_name
psbH
feature_type
gene
region
LSC
length_bp
222
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
21.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH_p1 AGACCACAATGGATCTATGCT CGAACAAAAAGCTAGGACTTCA 42.9 40.9 56.5 56.9 347 bp 1.0 40.0
psbH_p2 AGACCACAATGGATCTATGCT TCGAACAAAAAGCTAGGACTTCA 42.9 39.1 56.5 58.3 348 bp 1.0 40.0
psbH_p3 AGACCACAATGGATCTATGCT TCGAACAAAAAGCTAGGACTTC 42.9 40.9 56.5 56.7 348 bp 1.0 40.0
psbH_p4 AAGACCACAATGGATCTATGCT CGAACAAAAAGCTAGGACTTCA 40.9 40.9 57.2 56.9 348 bp 1.0 40.0
psbH_p5 AGACCACAATGGATCTATGCT TCGAACAAAAAGCTAGGACT 42.9 40.0 56.5 54.6 348 bp 1.0 40.0