Hotspot

aptA

LSC · 9584-11107 · 1524 bp

Back to Raphanus

Pi 0.000437

MarkerSeek score 38.40

Primer available yes

Label aptA

Feature Metadata

label_name: aptA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
aptA_p1	AGCCTTCCAAGCTAACGATG	TCATGTTTGGTTCGGGAAGG	50.0	50.0	58.0	58.1	2538-2543 bp	1.0	79.1
aptA_p2	ACCCTTAGCCTTCCAAGCTA	TCATGTTTGGTTCGGGAAGG	50.0	50.0	58.0	58.1	2544-2549 bp	1.0	79.1
aptA_p3	AGCCTTCCAAGCTAACGATG	AATTAGAAAACGCCCGAGCT	50.0	45.0	58.0	57.9	2386-2391 bp	1.0	79.0
aptA_p4	ACCCTTAGCCTTCCAAGCTA	AATTAGAAAACGCCCGAGCT	50.0	45.0	58.0	57.9	2392-2397 bp	1.0	79.0
aptA_p5	CGACGTCGACTAAAACCCTT	TCATGTTTGGTTCGGGAAGG	50.0	50.0	57.9	58.1	2558-2563 bp	1.0	78.9