Hotspot

accD

LSC · 55960-57435 · 1476 bp

Back to Raphanus

Pi 0.00226

MarkerSeek score 47.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 5
pi: 0.00226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGTAAATAGAGGCTAGAAGCTCT	TGAATTTTTCAGGGTTTTCGT	39.1	33.3	56.3	54.5	1543 bp	1.0	40.3
accD_p2	TGTAAATAGAGGCTAGAAGCTCT	TCAGGGTTTTCGTTTAATTCTGA	39.1	34.8	56.3	56.4	1535 bp	1.0	40.3
accD_p3	TGTAAATAGAGGCTAGAAGCTCT	TGAATTTTTCAGGGTTTTCGTT	39.1	31.8	56.3	55.3	1543 bp	1.0	40.3
accD_p4	TCATGTAAATAGAGGCTAGAAGCT	TGAATTTTTCAGGGTTTTCGT	37.5	33.3	57.0	54.5	1546 bp	1.0	40.3
accD_p5	CATGTAAATAGAGGCTAGAAGCTCT	TGAATTTTTCAGGGTTTTCGT	40.0	33.3	58.0	54.5	1545 bp	1.0	40.3