Hotspot

rpl32-trnL-UAG

SSC · 112561-113219 · 659 bp

Back to Raphanus

Pi 0.00506
MarkerSeek score 49.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
659
variable_sites
5
pi
0.00506
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTGGACATCGTTGAAAGCGA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 783-791 bp 1.0 78.5
rpl32-trnL-UAG_p2 TTGGACATCGTTGAAAGCGA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 776-784 bp 1.0 78.0
rpl32-trnL-UAG_p3 TTGGACATCGTTGAAAGCGA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 782-790 bp 1.0 77.9
rpl32-trnL-UAG_p4 TTGGACATCGTTGAAAGCGA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 781-789 bp 1.0 77.9
rpl32-trnL-UAG_p5 TTGGACATCGTTGAAAGCGA GAGCAGCGTGTCTACCAATT 45.0 50.0 58.1 58.3 777-785 bp 1.0 77.3