Hotspot

ndhF-rpl32

SSC · 111592-112401 · 810 bp

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Pi 0.00329

MarkerSeek score 48.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 810
variable_sites: 4
pi: 0.00329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGTAGAAGTCCAGCTCCGA	TCGCTTTCAACGATGTCCAA	50.0	45.0	57.8	58.1	988-989 bp	1.0	77.6
ndhF-rpl32_p2	AGAAGTCCAGCTCCGAGTAA	TCGCTTTCAACGATGTCCAA	50.0	45.0	57.8	58.1	984-985 bp	1.0	77.6
ndhF-rpl32_p3	AGTAGAAGTCCAGCTCCGAG	TCGCTTTCAACGATGTCCAA	55.0	45.0	58.2	58.1	987-988 bp	1.0	77.4
ndhF-rpl32_p4	GTAGAAGTCCAGCTCCGAGT	TCGCTTTCAACGATGTCCAA	55.0	45.0	58.5	58.1	986-987 bp	1.0	75.0
ndhF-rpl32_p5	AGAAGTCCAGCTCCGAGTAA	CGCTTTCAACGATGTCCAAT	50.0	45.0	57.8	57.1	983-984 bp	1.0	71.4