Hotspot

trnF-GAA-ndhJ

LSC · 47136-47730 · 595 bp

Back to Raphanus

Pi 0.00336

MarkerSeek score 48.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 3
pi: 0.00336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	707-708 bp	1.0	79.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	706-707 bp	1.0	79.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	704-705 bp	1.0	78.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	710-711 bp	1.0	77.1
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	CCGAAAGTTGGATAGGGTGG	50.0	55.0	57.5	58.0	709-710 bp	1.0	76.4