Hotspot

psaA-ycf3

LSC · 41071-41805 · 735 bp

Back to Raphanus

Pi 0.000907

MarkerSeek score 47.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 1
pi: 0.000907
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	847-848 bp	1.0	79.3
psaA-ycf3_p2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	864-865 bp	1.0	79.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	855-856 bp	1.0	77.3
psaA-ycf3_p4	AGAAATGACCGGGTTTAGCC	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	872-873 bp	1.0	77.2
psaA-ycf3_p5	AGCCCATTCCTCGAAAGAAG	ATTGGTTGACGATCACGAGG	50.0	50.0	57.6	58.0	848-849 bp	1.0	76.9