Hotspot

trnC-GCA-petN

LSC · 27107-27718 · 612 bp

Back to Raphanus

Pi 0.00109
MarkerSeek score 35.90
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
612
variable_sites
1
pi
0.00109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 AATAATTTTGGCGGCATGGC AGCCCATGCGAGACTTACTA 45.0 50.0 58.0 58.2 719 bp 1.0 77.9
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCATGCGAGACTTACTA 50.0 50.0 58.1 58.2 668 bp 1.0 77.7
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCATGCGAGACTTACTA 50.0 50.0 58.4 58.2 686 bp 1.0 75.5
trnC-GCA-petN_p4 AATAATTTTGGCGGCATGGC ATTAAACCAGCCCATGCGAG 45.0 50.0 58.0 58.6 727 bp 1.0 74.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG ATTAAACCAGCCCATGCGAG 50.0 50.0 58.1 58.6 676 bp 1.0 74.6