Hotspot

ndhC-trnV-UAC

LSC · 51626-52946 · 1321 bp

Back to Ranunculus

Pi 0.0542

MarkerSeek score 79.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1321
variable_sites: 216
pi: 0.0542
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCATTCCAACGCTCCTTTT	AGGAAAGAGCCCAATGCAAA	45.0	45.0	58.0	58.0	1670-1836 bp	1.0	91.9
ndhC-trnV-UAC_p2	AACAAAAACGAGCGCAAACA	CGCCAATGCTTTTTCAGAGG	40.0	50.0	58.0	58.0	2012-2143 bp	0.5	68.9
ndhC-trnV-UAC_p3	AACAACAAAAACGAGCGCAA	CGCCAATGCTTTTTCAGAGG	40.0	50.0	58.0	58.0	2015-2146 bp	0.5	68.9
ndhC-trnV-UAC_p4	ACAACAAAAACGAGCGCAAA	CGCCAATGCTTTTTCAGAGG	40.0	50.0	58.0	58.0	2014-2145 bp	0.5	68.9
ndhC-trnV-UAC_p5	ACCATTCCAACGCTCCTTTT	CGCCAATGCTTTTTCAGAGG	45.0	50.0	58.0	58.0	2160-2291 bp	0.5	68.7