Hotspot

trnT-GGU-psbD

LSC · 31935-33414 · 1480 bp

Back to Ranunculus

Pi 0.0452

MarkerSeek score 81.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1480
variable_sites: 215
pi: 0.0452
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	TCTCCGTAACCAGTCATCCA	50.0	50.0	57.9	57.8	1519-1648 bp	1.0	89.4
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.3	57.8	1482-1611 bp	1.0	87.9
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	GGTCTCTCCGTAACCAGTCA	50.0	55.0	57.9	58.5	1523-1652 bp	1.0	87.5
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GGGGAAGAGCAATAGACCAG	50.0	55.0	57.9	57.4	1564-1693 bp	1.0	86.1
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGTCTCTCCGTAACCAGTCA	50.0	55.0	58.3	58.5	1486-1615 bp	1.0	86.1