Hotspot

petN-psbM

LSC · 28814-29710 · 897 bp

Back to Ranunculus

Pi 0.0579

MarkerSeek score 77.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 144
pi: 0.0579
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAA	47.6	40.0	57.8	55.6	886-1314 bp	1.0	62.9
petN-psbM_p2	AGTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAAA	47.6	38.1	57.8	56.3	886-1314 bp	1.0	61.1
petN-psbM_p3	AGTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAAAA	47.6	36.4	57.8	57.1	886-1314 bp	1.0	58.8
petN-psbM_p4	AGTATGGGGAAGAAGTGGAC	ACGTAAAAACGGTCAGTCAA	50.0	40.0	56.2	55.6	886-1314 bp	1.0	58.4
petN-psbM_p5	GTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAA	50.0	40.0	56.2	55.6	885-1313 bp	1.0	58.4