Hotspot

rps16-trnQ-UUG

LSC · 5795-6884 · 1090 bp

Back to Ranunculus

Pi 0.0668
MarkerSeek score 81.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1090
variable_sites
224
pi
0.0668
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACCGATCACGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1034-1257 bp 1.0 90.7
rps16-trnQ-UUG_p2 CAACCGATCACGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1035-1258 bp 1.0 89.7
rps16-trnQ-UUG_p3 AACCGATCACGTCCTTCAAG CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 1038-1261 bp 1.0 89.7
rps16-trnQ-UUG_p4 AATCCACAACCGATCACGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 1041-1264 bp 1.0 89.2
rps16-trnQ-UUG_p5 CAACCGATCACGTCCTTCAA CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 1039-1262 bp 1.0 88.6