primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CACATTCGTCTCCACTCAGG	ACGGGAATCAAGCATTCACA	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.200639	4226	4709	1682-1775	0.909091	86.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CACATTCGTCTCCACTCAGG	CTTGAAAAAGGCGCTCAACC	20	20	55.0	50.0	58.0	58.2	0.0	7.6	0.230855	4226	4709	1040-1124	0.909091	86.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CACATTCGTCTCCACTCAGG	ATTCTTTCTCGAGCCGTACG	20	20	55.0	50.0	58.0	57.8	0.0	8.7	0.192137	4226	4709	1241-1325	0.863636	85.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CACATTCGTCTCCACTCAGG	ACACGGGAATCAAGCATTCA	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.200639	4226	4709	1684-1777	0.636364	75.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CACATTCGTCTCCACTCAGG	TCACACGGGAATCAAGCATT	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.200639	4226	4709	1686-1779	0.636364	75.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACCGATCACGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.156387	5795	6884	1034-1257	1.000000	90.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CAACCGATCACGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.285346	5795	6884	1035-1258	1.000000	89.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACCGATCACGTCCTTCAAG	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	57.9	57.8	0.0	16.9	0.291639	5795	6884	1038-1261	1.000000	89.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AATCCACAACCGATCACGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.3	58.1	0.0	16.9	0.354090	5795	6884	1041-1264	1.000000	89.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAACCGATCACGTCCTTCAA	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.420597	5795	6884	1039-1262	1.000000	88.6
petN-psbM_p1	petN-psbM	1	AGTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAA	21	20	47.6	40.0	57.8	55.6	0.0	2.8	3.637839	28814	29710	886-1314	1.000000	62.9
petN-psbM_p2	petN-psbM	2	AGTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAAA	21	21	47.6	38.1	57.8	56.3	0.0	2.8	3.859505	28814	29710	886-1314	1.000000	61.1
petN-psbM_p3	petN-psbM	3	AGTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAAAA	21	22	47.6	36.4	57.8	57.1	0.0	2.8	4.150186	28814	29710	886-1314	1.000000	58.8
petN-psbM_p4	petN-psbM	4	AGTATGGGGAAGAAGTGGAC	ACGTAAAAACGGTCAGTCAA	20	20	50.0	40.0	56.2	55.6	0.0	2.8	4.204375	28814	29710	886-1314	1.000000	58.4
petN-psbM_p5	petN-psbM	5	GTATGGGGAAGAAGTGGACT	ACGTAAAAACGGTCAGTCAA	20	20	50.0	40.0	56.2	55.6	0.0	2.8	4.204375	28814	29710	885-1313	1.000000	58.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	31142	31862	834-909	1.000000	90.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.262716	31142	31862	799-874	1.000000	89.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	31142	31862	796-871	1.000000	89.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	31142	31862	775-850	1.000000	89.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	31142	31862	831-906	1.000000	89.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GCCCTTTTAACTCAGCGGTA	TCTCCGTAACCAGTCATCCA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.328919	31935	33414	1519-1648	1.000000	89.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.508188	31935	33414	1482-1611	1.000000	87.9
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GCCCTTTTAACTCAGCGGTA	GGTCTCTCCGTAACCAGTCA	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.561045	31935	33414	1523-1652	1.000000	87.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	GGGGAAGAGCAATAGACCAG	20	20	50.0	55.0	57.9	57.4	0.0	0.0	0.732873	31935	33414	1564-1693	1.000000	86.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGTCTCTCCGTAACCAGTCA	20	20	50.0	55.0	58.3	58.5	0.0	0.0	0.740314	31935	33414	1486-1615	1.000000	86.1
psaA-ycf3_p1	psaA-ycf3	1	TAATGGAACCAACCGGCAAA	TATACCGATAGCAGGGGGTG	20	20	45.0	55.0	58.0	58.1	0.0	0.4	0.070180	42447	43212	1648-1735	1.000000	91.4
psaA-ycf3_p2	psaA-ycf3	2	TAATGGAACCAACCGGCAAA	TGGTTGAAGATTACGAGGCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.007784	42447	43212	1271-1329	0.772727	82.6
psaA-ycf3_p3	psaA-ycf3	3	TAATGGAACCAACCGGCAAA	GTTGAAGATTACGAGGCGGT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.009172	42447	43212	1269-1327	0.772727	82.6
psaA-ycf3_p4	psaA-ycf3	4	ACTTTGGAGCAGCTTTGTGA	TGGTTGAAGATTACGAGGCG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.061720	42447	43212	1291-1349	0.772727	82.0
psaA-ycf3_p5	psaA-ycf3	5	ACTTTGGAGCAGCTTTGTGA	GTTGAAGATTACGAGGCGGT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.063108	42447	43212	1289-1347	0.772727	82.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	ACCATTCCAACGCTCCTTTT	AGGAAAGAGCCCAATGCAAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.018633	51626	52946	1670-1836	1.000000	91.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AACAAAAACGAGCGCAAACA	CGCCAATGCTTTTTCAGAGG	20	20	40.0	50.0	58.0	58.0	11.5	0.0	0.029330	51626	52946	2012-2143	0.500000	68.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AACAACAAAAACGAGCGCAA	CGCCAATGCTTTTTCAGAGG	20	20	40.0	50.0	58.0	58.0	11.5	0.0	0.029330	51626	52946	2015-2146	0.500000	68.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ACAACAAAAACGAGCGCAAA	CGCCAATGCTTTTTCAGAGG	20	20	40.0	50.0	58.0	58.0	11.5	0.0	0.029330	51626	52946	2014-2145	0.500000	68.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	ACCATTCCAACGCTCCTTTT	CGCCAATGCTTTTTCAGAGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.017926	51626	52946	2160-2291	0.500000	68.7
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	TTAAAGCCGCCAGGAGAAAA	20	20	50.0	45.0	57.9	57.7	6.4	0.0	0.331269	65402	66552	1278-1360	1.000000	89.3
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	TTAAAGCCGCCAGGAGAAAA	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.368884	65402	66552	1291-1373	1.000000	89.0
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	TTAAAGCCGCCAGGAGAAAA	20	20	45.0	45.0	58.2	57.7	0.0	0.0	0.450955	65402	66552	1287-1369	1.000000	88.4
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	TTAAAGCCGCCAGGAGAAAA	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.467286	65402	66552	1286-1368	1.000000	88.3
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	TAGTTAAAGCCGCCAGGAGA	20	20	50.0	50.0	57.9	58.4	6.4	0.0	0.487410	65402	66552	1281-1363	1.000000	88.1
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	TTGTGTTTTACTCCCCGCAA	AGAGGACTAGCCGACCTTAC	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.109916	109871	111350	2327-2564	0.181818	57.3
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	TTGTGTTTTACTCCCCGCAA	GAGGACTAGCCGACCTTACT	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.109916	109871	111350	2326-2563	0.181818	57.3
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	TTTGTGTTTTACTCCCCGCA	AGAGGACTAGCCGACCTTAC	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.109916	109871	111350	2328-2565	0.181818	57.3
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	TTTGTGTTTTACTCCCCGCA	GAGGACTAGCCGACCTTACT	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.109916	109871	111350	2327-2564	0.181818	57.3
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	GAACTTGGGTGTGGGTCTTT	GAGGACTAGCCGACCTTACT	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.123049	109871	111350	2425-2662	0.181818	57.0
ndhF-rpl32_p1	ndhF-rpl32	1	GTCGGGAAAAGGAGAAGACC	AAGAGCCTTCGTTGTAACCG	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.304214	113841	114726	1658-1728	0.181818	52.9
ndhF-rpl32_p2	ndhF-rpl32	2	CAAGAGGTCGTGTGAACCAA	AAGAGCCTTCGTTGTAACCG	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.259890	113841	114726	2077-2147	0.181818	52.2
ndhF-rpl32_p3	ndhF-rpl32	3	GATTTAGCAACCGCACCAAC	AAGAGCCTTCGTTGTAACCG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.230806	113841	114726	2288-2358	0.181818	52.0
ndhF-rpl32_p4	ndhF-rpl32	4	AGATTTAGCAACCGCACCAA	AAGAGCCTTCGTTGTAACCG	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.303707	113841	114726	2289-2359	0.181818	51.5
ndhF-rpl32_p5	ndhF-rpl32	5	GCAACCGCACCAACAAATAA	AAGAGCCTTCGTTGTAACCG	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.349529	113841	114726	2282-2352	0.181818	51.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAGAATGCGCGAATTAACCC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	13.5	0.0	0.392729	114889	115573	1125-1229	0.500000	67.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAGAATGCGCGAATTAACCC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	13.5	0.0	0.394099	114889	115573	1126-1230	0.500000	67.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAGAATGCGCGAATTAACCC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.6	57.9	13.5	0.0	0.509176	114889	115573	1135-1239	0.500000	66.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GAGAATGCGCGAATTAACCC	AAGCGCCGCATAACTCAATA	20	20	50.0	45.0	57.6	58.1	13.5	13.4	0.436794	114889	115573	1737-1840	0.454545	62.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAGAATGCGCGAATTAACCC	CCTTTTTCCGACGAATGGGA	20	20	50.0	50.0	57.6	58.2	13.5	0.0	0.568824	114889	115573	1418-1521	0.272727	55.6
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	GCCCAACTAACCAACCAAGA	TTGTGTTTTACTCCCCGCAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.064526	130588	132067	2463-2647	0.136364	45.2
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	GCCCAACTAACCAACCAAGA	TTTGTGTTTTACTCCCCGCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.064526	130588	132067	2464-2648	0.136364	45.2
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	GCCCAACTAACCAACCAAGA	GAACTTGGGTGTGGGTCTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.077659	130588	132067	2562-2746	0.136364	45.1
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GCCCAACTAACCAACCAAGA	TCAAAGCGAGCCCTTTCATT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.100594	130588	132067	2153-2337	0.136364	45.0
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GCCCAACTAACCAACCAAGA	TTACTCCCCGCAACTTTTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110048	130588	132067	2456-2640	0.136364	44.9