Hotspot

ycf1

SSC · 10700-16267 · 5568 bp

Back to Racinaea

Pi 0.0113

MarkerSeek score 57.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 89
pi: 0.0113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGTAATGTCTGATCTCGCA	TGGTTCTTCCCAGAATTTGTCT	45.0	40.9	56.4	57.5	247-289 bp	1.0	55.4
ycf1_p2	TGGTAATGTCTGATCTCGCA	AGAATCTTGGTTCTTCCCAGA	45.0	42.9	56.4	56.3	254-296 bp	1.0	53.0
ycf1_p3	ATGGTAATGTCTGATCTCGCA	TGGTTCTTCCCAGAATTTGTCT	42.9	40.9	56.9	57.5	248-290 bp	1.0	51.7
ycf1_p4	TGGTAATGTCTGATCTCGCAT	TGGTTCTTCCCAGAATTTGTCT	42.9	40.9	56.9	57.5	247-289 bp	1.0	51.7
ycf1_p5	TGGTAATGTCTGATCTCGCA	TGGTTCTTCCCAGAATTTGTC	45.0	42.9	56.4	56.1	247-289 bp	1.0	51.6