Hotspot

accD

LSC · 99934-101400 · 1467 bp

Back to Racinaea

Pi 0.0132

MarkerSeek score 59.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 29
pi: 0.0132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAAATAGGGGGCAGGAAGA	ACAAAATAACTGAATCTAGTGCT	50.0	30.4	58.0	54.0	1453-1519 bp	1.0	45.1
accD_p2	TCAAATAGGGGGCAGGAAGA	ACAAACAAAATAACTGAATCTAGTGC	50.0	30.8	58.0	57.0	1457-1523 bp	1.0	45.1
accD_p3	TCATCAAATAGGGGGCAGGA	ACAAAATAACTGAATCTAGTGCT	50.0	30.4	58.1	54.0	1456-1522 bp	1.0	45.1
accD_p4	TCATCAAATAGGGGGCAGGA	ACAAACAAAATAACTGAATCTAGTGC	50.0	30.8	58.1	57.0	1460-1526 bp	1.0	45.1
accD_p5	TCAAATAGGGGGCAGGAAGA	ACAAACAAAATAACTGAATCTAGTGCT	50.0	29.6	58.0	58.1	1457-1523 bp	1.0	45.1