Hotspot

ycf1-ndhF

IRa · 154232-154264 · 33 bp

Back to Racinaea

Pi 0.6

MarkerSeek score 63.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: IRa
length_bp: 33
variable_sites: 3
pi: 0.6
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.15
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TCTGGTTTGAAAAACCTTTTCT	TATGGGAGGTGGACGAATCT	31.8	50.0	54.6	57.5	120-148 bp	0.666667	32.5
ycf1-ndhF_p2	TCTGGTTTGAAAAACCTTTTCTT	TATGGGAGGTGGACGAATCT	30.4	50.0	55.3	57.5	120-148 bp	0.666667	32.5
ycf1-ndhF_p3	TCTGGTTTGAAAAACCTTTTCT	ATGGGAGGTGGACGAATCTC	31.8	55.0	54.6	58.9	119-147 bp	0.666667	32.5
ycf1-ndhF_p4	TTCTGGTTTGAAAAACCTTTTCT	TATGGGAGGTGGACGAATCT	30.4	50.0	55.3	57.5	121-149 bp	0.666667	32.4
ycf1-ndhF_p5	TTCTGGTTTGAAAAACCTTTTCTT	TATGGGAGGTGGACGAATCT	29.2	50.0	56.1	57.5	121-149 bp	0.666667	32.4