Hotspot

rpl22-rps19

IRa · 127482-127600 · 119 bp

Back to Racinaea

Pi 0.0224

MarkerSeek score 60.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRa
length_bp: 119
variable_sites: 4
pi: 0.0224
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACTTCTTTACCTGAACTTGAGT	TCTGACTTTCGCGAGACATG	36.4	50.0	55.0	58.0	193-200 bp	1.0	44.5
rpl22-rps19_p2	ACTTCTTTACCTGAACTTGAGTT	TCTGACTTTCGCGAGACATG	34.8	50.0	55.7	58.0	193-200 bp	1.0	44.5
rpl22-rps19_p3	ACTTCTTTACCTGAACTTGAGT	CTCTGACTTTCGCGAGACAT	36.4	50.0	55.0	57.7	194-201 bp	1.0	44.4
rpl22-rps19_p4	ACTTCTTTACCTGAACTTGAGT	CCTACTCTGACTTTCGCGAG	36.4	55.0	55.0	57.8	198-205 bp	1.0	44.3
rpl22-rps19_p5	ACTTCTTTACCTGAACTTGAGTT	CCTACTCTGACTTTCGCGAG	34.8	55.0	55.7	57.8	198-205 bp	1.0	44.3