Hotspot

atpI-rps2

LSC · 56970-57224 · 255 bp

Back to Racinaea

Pi 0.0105

MarkerSeek score 59.20

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 255
variable_sites: 4
pi: 0.0105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GTTGGCCTACTTCTACACCC	ATTTCGATTCCAGCCAACGA	55.0	45.0	57.6	57.9	436-437 bp	1.0	76.7
atpI-rps2_p2	GTTGGCCTACTTCTACACCC	TTTCGATTCCAGCCAACGAT	55.0	45.0	57.6	57.9	435-436 bp	1.0	76.7
atpI-rps2_p3	GTTGGCCTACTTCTACACCC	CAGCCAACGATGACGCTATA	55.0	50.0	57.6	57.9	426-427 bp	1.0	76.5
atpI-rps2_p4	GGCCTACTTCTACACCCGAT	ATTTCGATTCCAGCCAACGA	55.0	45.0	58.6	57.9	433-434 bp	1.0	75.2
atpI-rps2_p5	GGCCTACTTCTACACCCGAT	TTTCGATTCCAGCCAACGAT	55.0	45.0	58.6	57.9	432-433 bp	1.0	75.2