Hotspot

atpH-atpI

LSC · 55242-56225 · 984 bp

Back to Racinaea

Pi 0.0105

MarkerSeek score 60.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 15
pi: 0.0105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1009-1041 bp	1.0	83.5
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	1025-1057 bp	1.0	82.6
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1010-1042 bp	1.0	82.3
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1024-1056 bp	1.0	82.0
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	CGAATCCATGGAGGGTCATC	50.0	55.0	58.3	57.8	1019-1051 bp	1.0	81.7