Hotspot

ycf1

SSC · 129625-135075 · 5451 bp

Back to Quillaja

Pi 0.00385

MarkerSeek score 54.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 21
pi: 0.00385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTGCATCAACTTTTTGTTCA	CGGAAGCTCTAGATAAGGGA	33.3	50.0	54.8	55.5	262 bp	1.0	41.2
ycf1_p2	AACTGCATCAACTTTTTGTTCA	CGGAAGCTCTAGATAAGGGA	31.8	50.0	55.6	55.5	263 bp	1.0	41.2
ycf1_p3	ACTGCATCAACTTTTTGTTCAA	CGGAAGCTCTAGATAAGGGA	31.8	50.0	55.6	55.5	262 bp	1.0	41.2
ycf1_p4	ACTGCATCAACTTTTTGTTCA	CGGAAGCTCTAGATAAGGGAT	33.3	47.6	54.8	56.1	262 bp	1.0	41.2
ycf1_p5	ACTGCATCAACTTTTTGTTCAAA	CGGAAGCTCTAGATAAGGGA	30.4	50.0	56.3	55.5	262 bp	1.0	41.2